General description

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.Tsujisaka, Y., et al. 1958. Nature181, 770.Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.

Packaging

5 ku in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Unit Definition

One unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.

Warning

Toxicity: Standard Handling (A)